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16s rrna gene amplicon (454) pyrosequencing sequencing  (Pyrosequencing Inc)

 
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    Structured Review

    Pyrosequencing Inc 16s rrna gene amplicon (454) pyrosequencing sequencing
    Summary of microbiome studies performed on animal models of TB and TB patients, investigating the impact of M . tuberculosis infection on the host microbiome.
    16s Rrna Gene Amplicon (454) Pyrosequencing Sequencing, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/16s+rrna+gene+amplicon+(454)+pyrosequencing+sequencing/16s+rrna+gene+amplicons/pmc08049499-5-40-45
    Average 90 stars, based on 1 article reviews
    16s rrna gene amplicon (454) pyrosequencing sequencing - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Microbiome-immune interactions in tuberculosis"

    Article Title: Microbiome-immune interactions in tuberculosis

    Journal: PLoS Pathogens

    doi: 10.1371/journal.ppat.1009377

    Summary of microbiome studies performed on animal models of TB and TB patients, investigating the impact of M . tuberculosis infection on the host microbiome.
    Figure Legend Snippet: Summary of microbiome studies performed on animal models of TB and TB patients, investigating the impact of M . tuberculosis infection on the host microbiome.

    Techniques Used: Infection, Sequencing, Amplification, Illumina Sequencing, Mouse Assay, Control, Sampling, Expressing, Bacteria

    Summary of microbiome studies performed on animal models of TB and TB patients, investigating the impact of anti-TB treatment on the host microbiome.
    Figure Legend Snippet: Summary of microbiome studies performed on animal models of TB and TB patients, investigating the impact of anti-TB treatment on the host microbiome.

    Techniques Used: Sequencing, Amplification, Illumina Sequencing, Infection, Functional Assay, Control, Mouse Assay, Expressing

    Related Articles

    Injection:

    Article Title: Effects of different carriers on biogas production and microbial community structure during anaerobic digestion of cassava ethanol wastewater.
    Article Snippet: In this study, an anaerobic bioreactor (AB) with no added fillers (ABWF), a packed-bed bioreactor with a porous ceramic filler (ABCF), and another packed-bed bioreactor filled with graphite felt (ABGF) were established for anaerobic digestion of cassava ethanol wastewater.. The results showed that ABCF exhibited excellent wastewater treatment performance in a stable process that was superior to ABWF and ABGF, with the following characteristics: a high chemical oxygen demand (COD) removal efficiency of 98.06%, constant optimal pH value of 7.25, low average volatile fatty acids (VFAs) concentration of 3.69 mmol/L, and maximum biogas production of 3,200 mL/d at a total reactor volume of 3.46 L. Illumina MiSeq sequencing analysis revealed that differences existed among the microbial communities of the three ABs that were in accordance with the operational characteristics.. The ABCF system displayed maximum bacterial diversity, whereas the ABWF system exhibited moderate richness and the ABGF system possessed the lowest species richness.

    Article Title: Simultaneous removal of crude oil and heavy metals by highly adapted bacterial strain Cutibacterium sp. NL2 isolated from Algerian oilfield.
    Article Snippet: Investigating the ability of bacteria to simultaneously enhance hydrocarbon removal and reduce heavy metals’ toxicity is necessary to design more effective bioremediation strategies.. A bacterium (NL2 strain) isolated from an Algerian oilfield was cultivated on crude oil as sole carbon and energy sources.. Molecular analyses of the 16S rRNA gene sequence placed the strain within the Cutibacterium genera.

    Article Title: Spatial and Temporal Dynamics of Microorganisms Living Along Steep Energy Gradients and Implications for Ecology and Geologic Preservation in the Deep Biosphere
    Article Snippet: .. Diversity of Microbial Communities in Production and Injection \Waters of Algerian Oilfields Revealed by 16S rRNA Gene Amplicon 454 Pyrosequencing. ..

    Amplification:

    Article Title: Effects of different carriers on biogas production and microbial community structure during anaerobic digestion of cassava ethanol wastewater.
    Article Snippet: In this study, an anaerobic bioreactor (AB) with no added fillers (ABWF), a packed-bed bioreactor with a porous ceramic filler (ABCF), and another packed-bed bioreactor filled with graphite felt (ABGF) were established for anaerobic digestion of cassava ethanol wastewater.. The results showed that ABCF exhibited excellent wastewater treatment performance in a stable process that was superior to ABWF and ABGF, with the following characteristics: a high chemical oxygen demand (COD) removal efficiency of 98.06%, constant optimal pH value of 7.25, low average volatile fatty acids (VFAs) concentration of 3.69 mmol/L, and maximum biogas production of 3,200 mL/d at a total reactor volume of 3.46 L. Illumina MiSeq sequencing analysis revealed that differences existed among the microbial communities of the three ABs that were in accordance with the operational characteristics.. The ABCF system displayed maximum bacterial diversity, whereas the ABWF system exhibited moderate richness and the ABGF system possessed the lowest species richness.

    Article Title: Microbial Communities Powering Plant‐Microbial Fuel Cells: Diversity, Functions and Biotechnological Perspectives
    Article Snippet: 16S rRNA gene‐based methods: T‐RFLP, Sanger clone libraries, 454 pyrosequencing (bacteria) , Lin and Lu ( ) . .. 16S rRNA gene amplicon 454 pyrosequencing (bacteria), 16S rRNA gene Sanger‐based clone libraries (archaea) , Lu et al. ( ) . .. 16S rRNA gene amplicon 454 pyrosequencing , Ueoka et al. ( ) .

    Article Title: Simultaneous removal of crude oil and heavy metals by highly adapted bacterial strain Cutibacterium sp. NL2 isolated from Algerian oilfield.
    Article Snippet: Investigating the ability of bacteria to simultaneously enhance hydrocarbon removal and reduce heavy metals’ toxicity is necessary to design more effective bioremediation strategies.. A bacterium (NL2 strain) isolated from an Algerian oilfield was cultivated on crude oil as sole carbon and energy sources.. Molecular analyses of the 16S rRNA gene sequence placed the strain within the Cutibacterium genera.

    Article Title: Spatial and Temporal Dynamics of Microorganisms Living Along Steep Energy Gradients and Implications for Ecology and Geologic Preservation in the Deep Biosphere
    Article Snippet: .. Diversity of Microbial Communities in Production and Injection \Waters of Algerian Oilfields Revealed by 16S rRNA Gene Amplicon 454 Pyrosequencing. ..

    Article Title: Microbial Communities Powering Plant-Microbial Fuel Cells: Diversity, Functions and Biotechnological Perspectives.
    Article Snippet: .. Microbial communities analysis method References 16S rRNA gene- based DGGE band sequencing (Sanger) Kaku et al. (2008) 16S rRNA gene- based methods: DGGE, T- RFLP, Sanger clone libraries De Schamphelaire et al. (2010) 16S rRNA gene amplicon 454 pyrosequencing and qRTPCR; FISH- CLSM (rRNAtargeted probes) Timmers et al. (2012) 16S rRNA gene amplicon pyrosequencing, Illuminabased shotgun metagenomics (HiSeq) Kouzuma et al. (2013) qPCR targeting archaeal 16S rRNA genes (group- specific primers) Arends et al. (2014) 16S rRNA gene amplicon 454 pyrosequencing Ahn et al. (2014) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries (PMFC- A2) Cabezas et al. (2015) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries, 454 pyrosequencing (bacteria) Lin and Lu (2015) 16S rRNA gene amplicon 454 pyrosequencing (bacteria), 16S rRNA gene Sanger- based clone libraries (archaea) Lu et al. (2015) 16S rRNA gene amplicon 454 pyrosequencing Ueoka et al. (2016) 16S rRNA gene amplicon 454 pyrosequencing Tapia et al. (2017) Selective plating, biochemical identification Azri et al. (2018) Illumina 16S rRNA amplicon sequencing (HiSeq) Liu et al. (2018) Illumina 16S rRNA amplicon sequencing Sudirjo et al. (2019) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan and Yu (2021) Isolation and Sanger- based 16S rRNA sequencing Arulmani et al. (2021) Illumina 16S rRNA amplicon sequencing Tongphanpharn et al. (2023) (Continues) Microbial communities analysis method References Illumina 16S rRNA amplicon sequencing (MiSeq) Sarma et al. (2024) Illumina 16S rRNA amplicon sequencing (MiSeq) Chen et al. (2024) Illumina 16S rRNA amplicon sequencing (NovaSeq) Gan et al. (2025) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan et al. (2025) TABLE 2 | (Continued) 17517915, 2026, 2, D ow nloaded from https://envirom icro-journals.onlinelibrary.w iley.com /doi/10.1111/1751-7915.70310, W iley O nline L ibrary on [17/02/2026]. ..

    Bacteria:

    Article Title: Microbial Communities Powering Plant‐Microbial Fuel Cells: Diversity, Functions and Biotechnological Perspectives
    Article Snippet: 16S rRNA gene‐based methods: T‐RFLP, Sanger clone libraries, 454 pyrosequencing (bacteria) , Lin and Lu ( ) . .. 16S rRNA gene amplicon 454 pyrosequencing (bacteria), 16S rRNA gene Sanger‐based clone libraries (archaea) , Lu et al. ( ) . .. 16S rRNA gene amplicon 454 pyrosequencing , Ueoka et al. ( ) .

    Article Title: Microbial Communities Powering Plant-Microbial Fuel Cells: Diversity, Functions and Biotechnological Perspectives.
    Article Snippet: .. Microbial communities analysis method References 16S rRNA gene- based DGGE band sequencing (Sanger) Kaku et al. (2008) 16S rRNA gene- based methods: DGGE, T- RFLP, Sanger clone libraries De Schamphelaire et al. (2010) 16S rRNA gene amplicon 454 pyrosequencing and qRTPCR; FISH- CLSM (rRNAtargeted probes) Timmers et al. (2012) 16S rRNA gene amplicon pyrosequencing, Illuminabased shotgun metagenomics (HiSeq) Kouzuma et al. (2013) qPCR targeting archaeal 16S rRNA genes (group- specific primers) Arends et al. (2014) 16S rRNA gene amplicon 454 pyrosequencing Ahn et al. (2014) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries (PMFC- A2) Cabezas et al. (2015) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries, 454 pyrosequencing (bacteria) Lin and Lu (2015) 16S rRNA gene amplicon 454 pyrosequencing (bacteria), 16S rRNA gene Sanger- based clone libraries (archaea) Lu et al. (2015) 16S rRNA gene amplicon 454 pyrosequencing Ueoka et al. (2016) 16S rRNA gene amplicon 454 pyrosequencing Tapia et al. (2017) Selective plating, biochemical identification Azri et al. (2018) Illumina 16S rRNA amplicon sequencing (HiSeq) Liu et al. (2018) Illumina 16S rRNA amplicon sequencing Sudirjo et al. (2019) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan and Yu (2021) Isolation and Sanger- based 16S rRNA sequencing Arulmani et al. (2021) Illumina 16S rRNA amplicon sequencing Tongphanpharn et al. (2023) (Continues) Microbial communities analysis method References Illumina 16S rRNA amplicon sequencing (MiSeq) Sarma et al. (2024) Illumina 16S rRNA amplicon sequencing (MiSeq) Chen et al. (2024) Illumina 16S rRNA amplicon sequencing (NovaSeq) Gan et al. (2025) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan et al. (2025) TABLE 2 | (Continued) 17517915, 2026, 2, D ow nloaded from https://envirom icro-journals.onlinelibrary.w iley.com /doi/10.1111/1751-7915.70310, W iley O nline L ibrary on [17/02/2026]. ..

    other:

    Article Title: Microbiome-immune interactions in tuberculosis
    Article Snippet: Feces , Female Balb/c mice ( n = 5) infected with Mtb CDC1551 or Mtb H37Rv; preinfection samples from each group as control ( n = 3) , Decrease of Clostridiales (Lachnospiraceae, Ruminococcaceae families) and Bacteroidales orders. , n.d. , 16S rRNA gene amplicon (454) pyrosequencing sequencing; Silva database Δ ; QIIME° , [ ] .

    Article Title: The gut microbiome: A line of defense against tuberculosis development
    Article Snippet: Stool samples from PTB patients (n=18) , Stool samples from healthy controls (n=18) , decreased alpha-deversity , Bifidobacteriaceae , Ruminococcaceae , Bacteroidaceae , Faecalibacterium , Faecalibacterium prausnitzii decreased in patients; , 16S rRNA gene amplicon (454) pyrosequencing , ( ) .

    Article Title: The gut microbiome: A line of defense against tuberculosis development
    Article Snippet: Stool samples from new PTB patients (n=83) , Stool samples from healthy controls (n=31) , decreased alpha-deversity , Firmicutes , Actinobacteria , Clostridales , Bifidobacteriales , Bifidobacteriaceae , Lachnospiraceae , Ruminococcaceae , Marinifilaceae , Eggerhellaceae , Barnesiellaceae , Blautia , Roseburia, Bifidobacterium , undifined Ruminococcaceae , Fusicatenibacter , Romboutsia decreased in patients; , 16S rRNA gene amplicon (454) pyrosequencing , ( ) .

    Denaturing Gradient Gel Electrophoresis:

    Article Title: Microbial Communities Powering Plant-Microbial Fuel Cells: Diversity, Functions and Biotechnological Perspectives.
    Article Snippet: .. Microbial communities analysis method References 16S rRNA gene- based DGGE band sequencing (Sanger) Kaku et al. (2008) 16S rRNA gene- based methods: DGGE, T- RFLP, Sanger clone libraries De Schamphelaire et al. (2010) 16S rRNA gene amplicon 454 pyrosequencing and qRTPCR; FISH- CLSM (rRNAtargeted probes) Timmers et al. (2012) 16S rRNA gene amplicon pyrosequencing, Illuminabased shotgun metagenomics (HiSeq) Kouzuma et al. (2013) qPCR targeting archaeal 16S rRNA genes (group- specific primers) Arends et al. (2014) 16S rRNA gene amplicon 454 pyrosequencing Ahn et al. (2014) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries (PMFC- A2) Cabezas et al. (2015) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries, 454 pyrosequencing (bacteria) Lin and Lu (2015) 16S rRNA gene amplicon 454 pyrosequencing (bacteria), 16S rRNA gene Sanger- based clone libraries (archaea) Lu et al. (2015) 16S rRNA gene amplicon 454 pyrosequencing Ueoka et al. (2016) 16S rRNA gene amplicon 454 pyrosequencing Tapia et al. (2017) Selective plating, biochemical identification Azri et al. (2018) Illumina 16S rRNA amplicon sequencing (HiSeq) Liu et al. (2018) Illumina 16S rRNA amplicon sequencing Sudirjo et al. (2019) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan and Yu (2021) Isolation and Sanger- based 16S rRNA sequencing Arulmani et al. (2021) Illumina 16S rRNA amplicon sequencing Tongphanpharn et al. (2023) (Continues) Microbial communities analysis method References Illumina 16S rRNA amplicon sequencing (MiSeq) Sarma et al. (2024) Illumina 16S rRNA amplicon sequencing (MiSeq) Chen et al. (2024) Illumina 16S rRNA amplicon sequencing (NovaSeq) Gan et al. (2025) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan et al. (2025) TABLE 2 | (Continued) 17517915, 2026, 2, D ow nloaded from https://envirom icro-journals.onlinelibrary.w iley.com /doi/10.1111/1751-7915.70310, W iley O nline L ibrary on [17/02/2026]. ..

    Sequencing:

    Article Title: Microbial Communities Powering Plant-Microbial Fuel Cells: Diversity, Functions and Biotechnological Perspectives.
    Article Snippet: .. Microbial communities analysis method References 16S rRNA gene- based DGGE band sequencing (Sanger) Kaku et al. (2008) 16S rRNA gene- based methods: DGGE, T- RFLP, Sanger clone libraries De Schamphelaire et al. (2010) 16S rRNA gene amplicon 454 pyrosequencing and qRTPCR; FISH- CLSM (rRNAtargeted probes) Timmers et al. (2012) 16S rRNA gene amplicon pyrosequencing, Illuminabased shotgun metagenomics (HiSeq) Kouzuma et al. (2013) qPCR targeting archaeal 16S rRNA genes (group- specific primers) Arends et al. (2014) 16S rRNA gene amplicon 454 pyrosequencing Ahn et al. (2014) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries (PMFC- A2) Cabezas et al. (2015) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries, 454 pyrosequencing (bacteria) Lin and Lu (2015) 16S rRNA gene amplicon 454 pyrosequencing (bacteria), 16S rRNA gene Sanger- based clone libraries (archaea) Lu et al. (2015) 16S rRNA gene amplicon 454 pyrosequencing Ueoka et al. (2016) 16S rRNA gene amplicon 454 pyrosequencing Tapia et al. (2017) Selective plating, biochemical identification Azri et al. (2018) Illumina 16S rRNA amplicon sequencing (HiSeq) Liu et al. (2018) Illumina 16S rRNA amplicon sequencing Sudirjo et al. (2019) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan and Yu (2021) Isolation and Sanger- based 16S rRNA sequencing Arulmani et al. (2021) Illumina 16S rRNA amplicon sequencing Tongphanpharn et al. (2023) (Continues) Microbial communities analysis method References Illumina 16S rRNA amplicon sequencing (MiSeq) Sarma et al. (2024) Illumina 16S rRNA amplicon sequencing (MiSeq) Chen et al. (2024) Illumina 16S rRNA amplicon sequencing (NovaSeq) Gan et al. (2025) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan et al. (2025) TABLE 2 | (Continued) 17517915, 2026, 2, D ow nloaded from https://envirom icro-journals.onlinelibrary.w iley.com /doi/10.1111/1751-7915.70310, W iley O nline L ibrary on [17/02/2026]. ..

    Terminal Restriction Fragment Length Polymorphism:

    Article Title: Microbial Communities Powering Plant-Microbial Fuel Cells: Diversity, Functions and Biotechnological Perspectives.
    Article Snippet: .. Microbial communities analysis method References 16S rRNA gene- based DGGE band sequencing (Sanger) Kaku et al. (2008) 16S rRNA gene- based methods: DGGE, T- RFLP, Sanger clone libraries De Schamphelaire et al. (2010) 16S rRNA gene amplicon 454 pyrosequencing and qRTPCR; FISH- CLSM (rRNAtargeted probes) Timmers et al. (2012) 16S rRNA gene amplicon pyrosequencing, Illuminabased shotgun metagenomics (HiSeq) Kouzuma et al. (2013) qPCR targeting archaeal 16S rRNA genes (group- specific primers) Arends et al. (2014) 16S rRNA gene amplicon 454 pyrosequencing Ahn et al. (2014) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries (PMFC- A2) Cabezas et al. (2015) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries, 454 pyrosequencing (bacteria) Lin and Lu (2015) 16S rRNA gene amplicon 454 pyrosequencing (bacteria), 16S rRNA gene Sanger- based clone libraries (archaea) Lu et al. (2015) 16S rRNA gene amplicon 454 pyrosequencing Ueoka et al. (2016) 16S rRNA gene amplicon 454 pyrosequencing Tapia et al. (2017) Selective plating, biochemical identification Azri et al. (2018) Illumina 16S rRNA amplicon sequencing (HiSeq) Liu et al. (2018) Illumina 16S rRNA amplicon sequencing Sudirjo et al. (2019) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan and Yu (2021) Isolation and Sanger- based 16S rRNA sequencing Arulmani et al. (2021) Illumina 16S rRNA amplicon sequencing Tongphanpharn et al. (2023) (Continues) Microbial communities analysis method References Illumina 16S rRNA amplicon sequencing (MiSeq) Sarma et al. (2024) Illumina 16S rRNA amplicon sequencing (MiSeq) Chen et al. (2024) Illumina 16S rRNA amplicon sequencing (NovaSeq) Gan et al. (2025) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan et al. (2025) TABLE 2 | (Continued) 17517915, 2026, 2, D ow nloaded from https://envirom icro-journals.onlinelibrary.w iley.com /doi/10.1111/1751-7915.70310, W iley O nline L ibrary on [17/02/2026]. ..

    Fluorescence In Situ Hybridization:

    Article Title: Microbial Communities Powering Plant-Microbial Fuel Cells: Diversity, Functions and Biotechnological Perspectives.
    Article Snippet: .. Microbial communities analysis method References 16S rRNA gene- based DGGE band sequencing (Sanger) Kaku et al. (2008) 16S rRNA gene- based methods: DGGE, T- RFLP, Sanger clone libraries De Schamphelaire et al. (2010) 16S rRNA gene amplicon 454 pyrosequencing and qRTPCR; FISH- CLSM (rRNAtargeted probes) Timmers et al. (2012) 16S rRNA gene amplicon pyrosequencing, Illuminabased shotgun metagenomics (HiSeq) Kouzuma et al. (2013) qPCR targeting archaeal 16S rRNA genes (group- specific primers) Arends et al. (2014) 16S rRNA gene amplicon 454 pyrosequencing Ahn et al. (2014) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries (PMFC- A2) Cabezas et al. (2015) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries, 454 pyrosequencing (bacteria) Lin and Lu (2015) 16S rRNA gene amplicon 454 pyrosequencing (bacteria), 16S rRNA gene Sanger- based clone libraries (archaea) Lu et al. (2015) 16S rRNA gene amplicon 454 pyrosequencing Ueoka et al. (2016) 16S rRNA gene amplicon 454 pyrosequencing Tapia et al. (2017) Selective plating, biochemical identification Azri et al. (2018) Illumina 16S rRNA amplicon sequencing (HiSeq) Liu et al. (2018) Illumina 16S rRNA amplicon sequencing Sudirjo et al. (2019) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan and Yu (2021) Isolation and Sanger- based 16S rRNA sequencing Arulmani et al. (2021) Illumina 16S rRNA amplicon sequencing Tongphanpharn et al. (2023) (Continues) Microbial communities analysis method References Illumina 16S rRNA amplicon sequencing (MiSeq) Sarma et al. (2024) Illumina 16S rRNA amplicon sequencing (MiSeq) Chen et al. (2024) Illumina 16S rRNA amplicon sequencing (NovaSeq) Gan et al. (2025) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan et al. (2025) TABLE 2 | (Continued) 17517915, 2026, 2, D ow nloaded from https://envirom icro-journals.onlinelibrary.w iley.com /doi/10.1111/1751-7915.70310, W iley O nline L ibrary on [17/02/2026]. ..

    Confocal Laser Scanning Microscopy:

    Article Title: Microbial Communities Powering Plant-Microbial Fuel Cells: Diversity, Functions and Biotechnological Perspectives.
    Article Snippet: .. Microbial communities analysis method References 16S rRNA gene- based DGGE band sequencing (Sanger) Kaku et al. (2008) 16S rRNA gene- based methods: DGGE, T- RFLP, Sanger clone libraries De Schamphelaire et al. (2010) 16S rRNA gene amplicon 454 pyrosequencing and qRTPCR; FISH- CLSM (rRNAtargeted probes) Timmers et al. (2012) 16S rRNA gene amplicon pyrosequencing, Illuminabased shotgun metagenomics (HiSeq) Kouzuma et al. (2013) qPCR targeting archaeal 16S rRNA genes (group- specific primers) Arends et al. (2014) 16S rRNA gene amplicon 454 pyrosequencing Ahn et al. (2014) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries (PMFC- A2) Cabezas et al. (2015) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries, 454 pyrosequencing (bacteria) Lin and Lu (2015) 16S rRNA gene amplicon 454 pyrosequencing (bacteria), 16S rRNA gene Sanger- based clone libraries (archaea) Lu et al. (2015) 16S rRNA gene amplicon 454 pyrosequencing Ueoka et al. (2016) 16S rRNA gene amplicon 454 pyrosequencing Tapia et al. (2017) Selective plating, biochemical identification Azri et al. (2018) Illumina 16S rRNA amplicon sequencing (HiSeq) Liu et al. (2018) Illumina 16S rRNA amplicon sequencing Sudirjo et al. (2019) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan and Yu (2021) Isolation and Sanger- based 16S rRNA sequencing Arulmani et al. (2021) Illumina 16S rRNA amplicon sequencing Tongphanpharn et al. (2023) (Continues) Microbial communities analysis method References Illumina 16S rRNA amplicon sequencing (MiSeq) Sarma et al. (2024) Illumina 16S rRNA amplicon sequencing (MiSeq) Chen et al. (2024) Illumina 16S rRNA amplicon sequencing (NovaSeq) Gan et al. (2025) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan et al. (2025) TABLE 2 | (Continued) 17517915, 2026, 2, D ow nloaded from https://envirom icro-journals.onlinelibrary.w iley.com /doi/10.1111/1751-7915.70310, W iley O nline L ibrary on [17/02/2026]. ..

    Metagenomics:

    Article Title: Microbial Communities Powering Plant-Microbial Fuel Cells: Diversity, Functions and Biotechnological Perspectives.
    Article Snippet: .. Microbial communities analysis method References 16S rRNA gene- based DGGE band sequencing (Sanger) Kaku et al. (2008) 16S rRNA gene- based methods: DGGE, T- RFLP, Sanger clone libraries De Schamphelaire et al. (2010) 16S rRNA gene amplicon 454 pyrosequencing and qRTPCR; FISH- CLSM (rRNAtargeted probes) Timmers et al. (2012) 16S rRNA gene amplicon pyrosequencing, Illuminabased shotgun metagenomics (HiSeq) Kouzuma et al. (2013) qPCR targeting archaeal 16S rRNA genes (group- specific primers) Arends et al. (2014) 16S rRNA gene amplicon 454 pyrosequencing Ahn et al. (2014) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries (PMFC- A2) Cabezas et al. (2015) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries, 454 pyrosequencing (bacteria) Lin and Lu (2015) 16S rRNA gene amplicon 454 pyrosequencing (bacteria), 16S rRNA gene Sanger- based clone libraries (archaea) Lu et al. (2015) 16S rRNA gene amplicon 454 pyrosequencing Ueoka et al. (2016) 16S rRNA gene amplicon 454 pyrosequencing Tapia et al. (2017) Selective plating, biochemical identification Azri et al. (2018) Illumina 16S rRNA amplicon sequencing (HiSeq) Liu et al. (2018) Illumina 16S rRNA amplicon sequencing Sudirjo et al. (2019) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan and Yu (2021) Isolation and Sanger- based 16S rRNA sequencing Arulmani et al. (2021) Illumina 16S rRNA amplicon sequencing Tongphanpharn et al. (2023) (Continues) Microbial communities analysis method References Illumina 16S rRNA amplicon sequencing (MiSeq) Sarma et al. (2024) Illumina 16S rRNA amplicon sequencing (MiSeq) Chen et al. (2024) Illumina 16S rRNA amplicon sequencing (NovaSeq) Gan et al. (2025) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan et al. (2025) TABLE 2 | (Continued) 17517915, 2026, 2, D ow nloaded from https://envirom icro-journals.onlinelibrary.w iley.com /doi/10.1111/1751-7915.70310, W iley O nline L ibrary on [17/02/2026]. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Microbial Communities Powering Plant-Microbial Fuel Cells: Diversity, Functions and Biotechnological Perspectives.
    Article Snippet: .. Microbial communities analysis method References 16S rRNA gene- based DGGE band sequencing (Sanger) Kaku et al. (2008) 16S rRNA gene- based methods: DGGE, T- RFLP, Sanger clone libraries De Schamphelaire et al. (2010) 16S rRNA gene amplicon 454 pyrosequencing and qRTPCR; FISH- CLSM (rRNAtargeted probes) Timmers et al. (2012) 16S rRNA gene amplicon pyrosequencing, Illuminabased shotgun metagenomics (HiSeq) Kouzuma et al. (2013) qPCR targeting archaeal 16S rRNA genes (group- specific primers) Arends et al. (2014) 16S rRNA gene amplicon 454 pyrosequencing Ahn et al. (2014) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries (PMFC- A2) Cabezas et al. (2015) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries, 454 pyrosequencing (bacteria) Lin and Lu (2015) 16S rRNA gene amplicon 454 pyrosequencing (bacteria), 16S rRNA gene Sanger- based clone libraries (archaea) Lu et al. (2015) 16S rRNA gene amplicon 454 pyrosequencing Ueoka et al. (2016) 16S rRNA gene amplicon 454 pyrosequencing Tapia et al. (2017) Selective plating, biochemical identification Azri et al. (2018) Illumina 16S rRNA amplicon sequencing (HiSeq) Liu et al. (2018) Illumina 16S rRNA amplicon sequencing Sudirjo et al. (2019) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan and Yu (2021) Isolation and Sanger- based 16S rRNA sequencing Arulmani et al. (2021) Illumina 16S rRNA amplicon sequencing Tongphanpharn et al. (2023) (Continues) Microbial communities analysis method References Illumina 16S rRNA amplicon sequencing (MiSeq) Sarma et al. (2024) Illumina 16S rRNA amplicon sequencing (MiSeq) Chen et al. (2024) Illumina 16S rRNA amplicon sequencing (NovaSeq) Gan et al. (2025) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan et al. (2025) TABLE 2 | (Continued) 17517915, 2026, 2, D ow nloaded from https://envirom icro-journals.onlinelibrary.w iley.com /doi/10.1111/1751-7915.70310, W iley O nline L ibrary on [17/02/2026]. ..

    Isolation:

    Article Title: Microbial Communities Powering Plant-Microbial Fuel Cells: Diversity, Functions and Biotechnological Perspectives.
    Article Snippet: .. Microbial communities analysis method References 16S rRNA gene- based DGGE band sequencing (Sanger) Kaku et al. (2008) 16S rRNA gene- based methods: DGGE, T- RFLP, Sanger clone libraries De Schamphelaire et al. (2010) 16S rRNA gene amplicon 454 pyrosequencing and qRTPCR; FISH- CLSM (rRNAtargeted probes) Timmers et al. (2012) 16S rRNA gene amplicon pyrosequencing, Illuminabased shotgun metagenomics (HiSeq) Kouzuma et al. (2013) qPCR targeting archaeal 16S rRNA genes (group- specific primers) Arends et al. (2014) 16S rRNA gene amplicon 454 pyrosequencing Ahn et al. (2014) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries (PMFC- A2) Cabezas et al. (2015) 16S rRNA gene- based methods: T- RFLP, Sanger clone libraries, 454 pyrosequencing (bacteria) Lin and Lu (2015) 16S rRNA gene amplicon 454 pyrosequencing (bacteria), 16S rRNA gene Sanger- based clone libraries (archaea) Lu et al. (2015) 16S rRNA gene amplicon 454 pyrosequencing Ueoka et al. (2016) 16S rRNA gene amplicon 454 pyrosequencing Tapia et al. (2017) Selective plating, biochemical identification Azri et al. (2018) Illumina 16S rRNA amplicon sequencing (HiSeq) Liu et al. (2018) Illumina 16S rRNA amplicon sequencing Sudirjo et al. (2019) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan and Yu (2021) Isolation and Sanger- based 16S rRNA sequencing Arulmani et al. (2021) Illumina 16S rRNA amplicon sequencing Tongphanpharn et al. (2023) (Continues) Microbial communities analysis method References Illumina 16S rRNA amplicon sequencing (MiSeq) Sarma et al. (2024) Illumina 16S rRNA amplicon sequencing (MiSeq) Chen et al. (2024) Illumina 16S rRNA amplicon sequencing (NovaSeq) Gan et al. (2025) Illumina 16S rRNA amplicon sequencing (MiSeq) Guan et al. (2025) TABLE 2 | (Continued) 17517915, 2026, 2, D ow nloaded from https://envirom icro-journals.onlinelibrary.w iley.com /doi/10.1111/1751-7915.70310, W iley O nline L ibrary on [17/02/2026]. ..



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    Summary of microbiome studies performed on animal models of TB and TB patients, investigating the impact of M . tuberculosis infection on the host microbiome.

    Journal: PLoS Pathogens

    Article Title: Microbiome-immune interactions in tuberculosis

    doi: 10.1371/journal.ppat.1009377

    Figure Lengend Snippet: Summary of microbiome studies performed on animal models of TB and TB patients, investigating the impact of M . tuberculosis infection on the host microbiome.

    Article Snippet: Feces , Female Balb/c mice ( n = 5) infected with Mtb CDC1551 or Mtb H37Rv; preinfection samples from each group as control ( n = 3) , Decrease of Clostridiales (Lachnospiraceae, Ruminococcaceae families) and Bacteroidales orders. , n.d. , 16S rRNA gene amplicon (454) pyrosequencing sequencing; Silva database Δ ; QIIME° , [ ] .

    Techniques: Infection, Sequencing, Amplification, Illumina Sequencing, Mouse Assay, Control, Sampling, Expressing, Bacteria

    Summary of microbiome studies performed on animal models of TB and TB patients, investigating the impact of anti-TB treatment on the host microbiome.

    Journal: PLoS Pathogens

    Article Title: Microbiome-immune interactions in tuberculosis

    doi: 10.1371/journal.ppat.1009377

    Figure Lengend Snippet: Summary of microbiome studies performed on animal models of TB and TB patients, investigating the impact of anti-TB treatment on the host microbiome.

    Article Snippet: Feces , Female Balb/c mice ( n = 5) infected with Mtb CDC1551 or Mtb H37Rv; preinfection samples from each group as control ( n = 3) , Decrease of Clostridiales (Lachnospiraceae, Ruminococcaceae families) and Bacteroidales orders. , n.d. , 16S rRNA gene amplicon (454) pyrosequencing sequencing; Silva database Δ ; QIIME° , [ ] .

    Techniques: Sequencing, Amplification, Illumina Sequencing, Infection, Functional Assay, Control, Mouse Assay, Expressing